Info

Two day workshop

Time: November 27 and 28, 2024, 8:30 a.m.

Place: Genetic building (rooms 1.66; 1.70; 1.58); IPK campus

Trainer: Dr. Beate Kellert and Dr. Jörg Fuchs (QG/CSF)

Language: English


Content

Flow cytometry is a technology that allows the simultaneous measurement and subsequent analysis of multiple physical characteristics of single particles (e.g. cells or nuclei) in a fluid stream while passing a beam of light. This method has numerous applications in biomedical research, like immunology, haematology or oncology. In the last decades flow cytometry has become of increasing interest also for plant researchers and is now widely used for ploidy screenings, detection of mixoploidy and aneuploidy, cell cycle analysis, determination of reproduction pathways, and estimations of absolute nuclear DNA content.

After the course, you should have not only acquired basic knowledge about flow cytometry, but also about the various possibilities regarding this method at the IPK.


The workshop comprises

  • In the theoretical part of the course, you will learn about the basic principles of flow cytometry and its application in plant science.
  • With the focus on DNA flow cytometry the practical part includes setting up and controlling the cytometer, sample preparation, measurement, as well as data analysis and interpretation. We will cover genome size estimation, sorting of nuclei according to differences in the DNA content and semi-automated high throughput ploidy analysis. Plant material will be provided but you are also encouraged to bring your own material of interest (e.g. fresh leaf tissue or seeds).

Following on from the training

After the course, you should have not only acquired basic knowledge about flow cytometry, but also about the various possibilities regarding this method at the IPK.

If you are interested please register here until November 15, 2024. Application will be accepted in chronological order of receipt. Application will be accepted in chronological order of receipt.

Please note: The number of participants is limited to max. eight (8)